Recipe

Hybridization solution for ISH

Reagent Quantity (for 100 mL) Final concentration
Denhardt’s reagent (50X) 2 mL 1X
Dextran sulfate 10 g 10%
EDTA (0.5 M) 0.2 mL 1 mM
Formamide 50 mL 50%
NaCl (5 M) 12 mL 600 mM
SDS (20%) 1.25 mL 0.25%
Tris-Cl (1 M, pH 7.5) 1 mL 10 mM
tRNA from yeast (GIBCO) 20 mg 200 μg/mL
H2O to 100 mL
Store at -20°C.

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  1. doi:10.1101/pdb.rec11714 Cold Spring Harb Protoc 2009: pdb.rec11714-
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