Recipe

Hybridization buffer for microarray analysis

Reagent Quantity (for 10 mL) Final concentration
Denhardt’s reagent (50X) 1 mL 5X
Formamide, deionized 5 mL 50%
Potassium phosphate (0.1 M) 0.5 mL 5 mM
SDS (10%) 0.5 mL 0.5%
SSC (20X) 2.5 mL 5X
H2O 0.5 mL

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  1. doi:10.1101/pdb.rec11716 Cold Spring Harb Protoc 2009: pdb.rec11716-
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