Recipe

EB extraction buffer

100 mM Tris-Cl (pH 8.0)

50 mM EDTA (pH 8.0)

500 mM NaCl

10 mM β-mercaptoethanol

Add β-mercaptoethanol just before use: 7.2 μL/10 mL.

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  1. doi:10.1101/pdb.rec11720 Cold Spring Harb Protoc 2009: pdb.rec11720-
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