Cover image

Cover Illustration: Multicolor labeling of embryonic day 14 mouse cortex using seven combinations of the carbocyanine dyes DiI, DiO, and DiD delivered ballistically. The brain was fixed with paraformaldehyde, sectioned by a vibratome, and then ballistically labeled using a gene gun. (Image reproduced from Gan, W.B., Grutzendler, J., Wong, W.T., Wong, R.O.L., and Lichtman, J.W. 2000. Multicolor “DiOlistic” labeling of neuronal circuits using lipophilic dye combinations. Neuron 27: 219-225. Permission to reproduce granted by Elsevier © 2000.) For more information on the methods used, see Ballistic Delivery of Dyes for Structural and Functional Studies of the Nervous System (doi:10.1101/pdb.prot5202).