Induction of Larval Metamorphosis in the Snail Ilyanassa
- Maey Gharbiah1,
- James Cooley1,
- Esther M. Leise2,
- Ayaki Nakamoto1,
- Jeremy S. Rabinowitz3,
- J. David Lambert3,4 and
- Lisa M. Nagy1,4
- 1 Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
- 2 Department of Biology, University of North Carolina, Greensboro, NC 27402-6170, USA
- 3 Department of Biology, University of Rochester, Rochester, NY 14627, USA
- ↵4Corresponding authors (dlamber2{at}mail.rochester.edu; lnagy{at}u.arizona.edu)
INTRODUCTION
The marine gastropod Ilyanassa obsoleta is a long-standing and very useful model for studies of embryonic development. It is an especially important model for spiralian development, and for studies of asymmetric cell division. The embryos are amenable to classic embryological manipulation techniques as well as a growing number of molecular approaches. Ilyanassa is also an important model for studies of metamorphosis, the ecology of parasitism, the effects of environmental contaminants on morphology and sexual function, and comparative neurobiology. Ilyanassa adults are readily obtainable and easy to keep in the laboratory, and they can produce high-quality embryos nearly year-round in the laboratory. After hatching from capsules, larval Ilyanassa can be maintained in culture, feeding on single-celled algae. The larvae will become competent to undergo metamorphosis after ~3 wk in culture. Metamorphosis can be induced artificially by treating with either the neurotransmitter serotonin or the nitric oxide synthase inhibitor 7-nitroindazole. Both of these reagents have been shown to induce metamorphosis in >75% of larvae within 48 h. This protocol describes the induction of metamorphosis in snail larvae.
