Arnolds’ Silver Staining of Helbodella (Leech) Embryos

David A. Weisblat; Dian-Han Kuo

doi:10.1101/pdb.prot5192

Protocol

Arnolds’ Silver Staining of Helbodella (Leech) Embryos

David A. Weisblat1 and
Dian-Han Kuo

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA

↵¹Corresponding author (weisblat{at}berkeley.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

Arnolds’ silver staining is used to visualize the boundaries of epidermal cells at the surface of embryos. As described in this article, silver staining can be performed on either living or fixed Helbodella (leech) embryos. To preserve the cell junction in fixed embryos, a special fixation procedure is used.