In Situ Hybridization of Helobdella (Leech) Embryos

David A. Weisblat; Dian-Han Kuo

doi:10.1101/pdb.prot5194

Protocol

In Situ Hybridization of Helobdella (Leech) Embryos

David A. Weisblat1 and
Dian-Han Kuo

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA

↵¹Corresponding author (weisblat{at}berkeley.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

The purpose of this protocol is to reveal the localization of transcripts in the Helobdella (leech) embryo. In situ hybridization protocols for Helobdella embryos are derived from those used for zebrafish and Xenopus. The protocols are different for early- and late-stage embryos (stages 1-8 and stages 9-11, respectively) due to a difference in tissue permeability.