Quick Preparation of Genomic DNA from Drosophila
Adapted from Drosophila Protocols (eds. Sullivan et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.INTRODUCTION
This protocol describes a standard miniprep for Drosophila melanogaster that requires very few flies and produces high-quality DNA. This method can also be used to isolate RNA when RNase-free conditions are utilized; an extra step must be taken to rid the sample of genomic DNA (e.g., RNase-free DNase digestion).
