Recipe

AM master mix

Reagent Quantity
ATP (0.5 M) 3 μL
Creatine phosphate (0.5 M) 30 μL
H2O 16.5 μL
MgCl2 (100 mM) 25 μL
Immediately before use, add:
Creatine kinase solution (5 mg/mL) 0.5 μL

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  1. doi:10.1101/pdb.rec11754 Cold Spring Harb Protoc 2009: pdb.rec11754-
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