Recipe

NAP1 purification buffers

Reagent Quantity (per 1 L)
0.1 M NaCl 0.2 M NaCl 0.5 M NaCl
NaCl (5 M) 20 mL 40 mL 100 mL
Nonidet P-40 (10%, v/v) 1 mL 1 mL 1 mL
Adjust the volume to 1 L with:
Buffer R+
Store for up to 24 h at 4°C.

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  1. doi:10.1101/pdb.rec11764 Cold Spring Harb Protoc 2009: pdb.rec11764-

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