Recipe

Dilution buffer F

10% (v/v) glycerol

0.02% (v/v) Nonidet P-40

20 mM Tris-Cl (pH 7.9)

Store solution for up to 24 h at 4°C.

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  1. doi:10.1101/pdb.rec11768 Cold Spring Harb Protoc 2009: pdb.rec11768-
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