Recipe

Ligase buffer (10X)

0.5 M Tris-Cl (pH 7.5)

100 mM MgCl2

100 mM DTT (dithiothreitol)

10 mM ATP

0.25 mg/mL bovine serum albumin (BSA)

Store at −20ºC.

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  1. doi:10.1101/pdb.rec11772 Cold Spring Harb Protoc 2009: pdb.rec11772-
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