Recipe

Standard external saline

2 mm CaCl2

20 mm glucose

2.5 mm KCl

1 mm MgCl2

125 mm NaCl

26 mm NaHCO3

1.25 mm NaH2PO4

Aerate with 95% O2 and 5% CO2. The pH is ~7.3 at 21°C.

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  1. doi:10.1101/pdb.rec11773 Cold Spring Harb Protoc 2009: pdb.rec11773-
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