Dissection of Larval and Pupal Wings from the African Butterfly Bicyclus anynana

Paul M. Brakefield; Patrícia Beldade; Bas J. Zwaan

doi:10.1101/pdb.prot5207

Protocol

Dissection of Larval and Pupal Wings from the African Butterfly Bicyclus anynana

Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

↵¹Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

This protocol describes a procedure for the dissection of larval and pupal wings from the African butterfly Bicyclus anynana. Dissected pupal wings can be used subsequently for in situ hybridization or for immunohistochemistry. They can also be analyzed with stereomicroscopy to study scale maturation and pigment deposition.