Immunohistochemistry Staining of Wing Discs from the African Butterfly Bicyclus anynana

Paul M. Brakefield; Patrícia Beldade; Bas J. Zwaan

doi:10.1101/pdb.prot5210

Protocol

Immunohistochemistry Staining of Wing Discs from the African Butterfly Bicyclus anynana

Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

↵¹Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

This protocol describes an immunohistochemical method for the detection of proteins in developing wings of the African butterfly Bicyclus anynana. The wing discs are stained with a primary antibody against a protein of interest, followed by a fluorescently labeled secondary antibody.