Recipe

Embryonic medium

Reagent Quantity (for 1 L) Final concentration
NaCl 0.87 g 15 mM
KCl 0.04 g 0.5 mM
CaCl2•2H2O 0.15 g 1 mM
MgSO4•7H2O 0.25 g 1 mM
KH2PO4 0.02 g 0.15 mM
Na2HPO4•12H2O 0.02 g 0.05 mM
NaHCO3 0.06 g 0.7 mM
Adjust the final volume to 1 L with H2O. Adjust the pH to 7.0-7.5.

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  1. doi:10.1101/pdb.rec11776 Cold Spring Harb Protoc 2009: pdb.rec11776-
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