Staining buffer (SBB)
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Tris-Cl (1 M, pH 9.5) | 10 mL | 100 mM |
| NaCl (100 mM) | 100 μL | 0.1 mM |
| Tween 20 | 100 μL | 0.1% (v/v) |
| Adjust the final volume to 100 mL with H2O. Prepare fresh for each use from stock solutions. | ||
