Recipe

Staining solution (SS)

Reagent Quantity Final concentration
Staining buffer (SBB) 1 mL 1X
NBT/BCIP stock solution (Roche) 10 μL
NBT/BCIP stock solution contains equal parts of:
NBT
BCIP
Prepare fresh and protect from the light.

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  1. doi:10.1101/pdb.rec11789 Cold Spring Harb Protoc 2009: pdb.rec11789-
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