Recipe

Block buffer for wing discs (BBW)

Reagent Quantity (for 100 mL) Final concentration
Tris-Cl (1 M, pH 6.8) 5 mL 50 mM
NaCl (1 M) 15 mL 150 mM
IGEPAL CA-630 500 μL 0.5% (v/v)
Bovine serum albumin 500 mg 5 mg/mL

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  1. doi:10.1101/pdb.rec11790 Cold Spring Harb Protoc 2009: pdb.rec11790-
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