Recipe

Fix buffer for wing discs (FBW)

Reagent Quantity (for 100 mL) Final concentration
PIPES (1 M, pH 6.9) 10 mL 0.1 M
EGTA (100 mM, pH 6.9) 1 mL 1 mM
Triton X-100 1 mL 1% (v/v)
MgSO4 (1 M) 200 μL 2 mM
Formaldehyde (37%) Just before use, add 1 part of 37% formaldehyde to 3 parts of the solution.

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  1. doi:10.1101/pdb.rec11791 Cold Spring Harb Protoc 2009: pdb.rec11791-
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