Subbing solution
Chrome alum
Gelatin
Poly-L-lysine (m.w. >300,000; Sigma)
METHOD
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1. Bring 200 mL of H2O to 60°C.
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2. Add 0.4 g of gelatin and cool to 40°C.
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3. Add 0.04 g of chrome alum.
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4. Add sodium azide to 1 mM.
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5. Add poly-L-lysine to 1 mg/mL.
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6. Put the subbing solution in a Coplin jar and store it at 4°C.
