Worm acetone powder

MATERIALS

METHOD

• 1. Homogenize worms in Dounce homogenizer; use ~1 g of worms/mL of M9 buffer.

• 2. Set on ice for 5 min.

• 3. Add 4 mL of −20°C acetone/mL of worm suspension. Mix vigorously.

• 4. Set on ice for 30 min with occasional vigorous mixing.

• 5. Centrifuge at 10,000g for 10 min.

• 6. Resuspend pellet with fresh −20°C acetone.

• 7. Mix vigorously on ice for 10 min.

• 8. Centrifuge at 10,000g for 10 min.

• 9. Spread pellet on clean filter paper and allow to dry at room temperature.

• 10. When dry, break up chunks in a mortar and pestle.

• 11. Transfer powder to a microcentrifuge tube and store at 4°C.