Recipe

Cathode buffer (5X)

75 mM Bis-Tris

0.1% (w/v) Coomassie brilliant blue G250

250 mM tricine

Adjust the pH to 7.0 at 4°C with HCl.

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  1. doi:10.1101/pdb.rec11818 Cold Spring Harb Protoc 2009: pdb.rec11818-
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