Recipe

Cathode buffer, second dimension

0.1 % (w/v) SDS (sodium dodecyl sulfate)

0.1 M tricine

0.1 M Tris

The pH is 8.25; no adjustment is needed.

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  1. doi:10.1101/pdb.rec11819 Cold Spring Harb Protoc 2009: pdb.rec11819-
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