| 0.1 M cytosine (Sigma-Aldrich C3506)
|
10 mL |
5.6 mM |
| 0.1 M L-arginine (Sigma-Aldrich A8094) |
10 mL |
5.6 mM |
| 0.1 M L-aspartic acid (Sigma-Aldrich A6558) |
10 mL |
5.6 mM |
| 0.1 M citric acid monohydrate (Sigma-Aldrich C7129)
|
10 mL |
5.6 mM |
| 0.1 M L-glutamic acid (Sigma-Aldrich G1626) |
10 mL |
5.6 mM |
| 0.1 M L-histidine (Sigma-Aldrich H8000) |
10 mL |
5.6 mM |
| 0.1 M L-leucine (Sigma-Aldrich L8912) |
10 mL |
5.6 mM |
| 0.1 M L-lysine (Sigma-Aldrich L5501) |
10 mL |
5.6 mM |
| 0.1 M L-malic acid (Sigma-Aldrich M1000)
|
10 mL |
5.6 mM |
| 0.1 M L-methionine (Sigma-Aldrich M9625) |
10 mL |
5.6 mM |
| 0.1 M L-serine (Sigma-Aldrich S4500) |
10 mL |
5.6 mM |
| 0.1 M L-threonine (Sigma-Aldrich T8625) |
10 mL |
5.6 mM |
| 0.1 M L-tryptophan (Sigma-Aldrich T0254)
|
10 mL |
5.6 mM |
| 0.1 M L-valine (Sigma-Aldrich V0500)
|
10 mL |
5.6 mM |
| 0.1 M pyruvic acid (Sigma-Aldrich P1656) |
10 mL |
5.6 mM |
| 0.1 M sodium fumarate dibasic (Sigma-Aldrich F1506)
|
10 mL |
5.6 mM |
| H2O, ultrapure
|
20 mL |
5.6 mM |
| Sodium succinate dibasic hexahydrate (Sigma-Aldrich S2378)
|
0.27 g |
5.6 mM |
| Uracil (Sigma-Aldrich U0750) |
0.11 g |
5.6 mM |
|
| Prepare 0.1-M stock solutions of each component except for the sodium succinate dibasic hexahydrate and uracil by dissolving the appropriate amount of compound in 50 mL of ultrapure
H2O and filter-sterilizing. (Sodium succinate dibasic hexahydrate and uracil do not dissolve readily in 50 mL.)
|
| To prepare the cocktail, combine 10 mL of each stock solution with 20 mL of ultrapure H2O (total volume = 180 mL). Add the sodium succinate dibasic hexahydrate and uracil as powders. Stir to dissolve, and filter-sterilize.
|
| The cocktail is used to supplement yeast metabolic footprinting (YMF) medium at a ratio of 9 mL cocktail to 41 mL medium,
such that each component of the cocktail is at a final concentration of 1 mM.
|
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