Whole-Mount In Situ Hybridization of Honeybee (Apis mellifera) Tissues
- Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand
- ↵1Corresponding author (peter.dearden{at}otago.ac.nz)
INTRODUCTION
This protocol outlines how to perform in situ hybridization on honeybee embryos, adult brains, or ovaries to detect the expression pattern of a targeted transcript. The tissue is digested by proteinase K to promote probe penetration, and then prehybridized to block nonspecific binding of the probe.
