Protocol

Immunohistochemistry on Honeybee (Apis mellifera) Embryos

  1. Megan J. Wilson
  1. Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand
  1. ↵1Corresponding author (peter.dearden{at}otago.ac.nz)

INTRODUCTION

This protocol describes the preparation and staining of honeybee embryos for immunohistochemistry. An antigen retrieval step is required in order to restore antigens that have become masked by the long fixation step required for honeybee embryos.

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  1. doi:10.1101/pdb.prot5227 Cold Spring Harb Protoc 2009: pdb.prot5227-
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