Recipe

FA lysis buffer

EDTA (1 mM, pH 8.0)

HEPES-KOH (50 mM, pH 7.5)

NaCl (140 mM)

Protease inhibitors (100X), diluted to 1X final concentration

Sodium deoxycholate (0.1%, w/v)

Triton X-100 (1%, v/v)

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  1. doi:10.1101/pdb.rec11842 Cold Spring Harb Protoc 2009: pdb.rec11842-
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