Recipe

PAC2 medium

Leibovitz’s L-15 medium, liquid, with L-glutamine (1X; GIBCO/Invitrogen)

Fetal bovine serum, EU-approved (FBS; e.g., GIBCO/Invitrogen or PAA A15-101)

MEM nonessential amino acids, without L-glutamine, liquid (100X; GIBCO/Invitrogen)

Penicillin-streptomycin, liquid (GIBCO/Invitrogen)

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  1. doi:10.1101/pdb.rec11847 Cold Spring Harb Protoc 2009: pdb.rec11847-
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