Recipe

IPN150

MgCl2 (5 mM)

NaCl (150 mM)

Nonidet P-40 (0.1%, v/v)

Tris-Cl (50 mM; pH 7.6)

Immediately before use, add:

DTT (Dithiothreitol) (1 mM)

Phenylmethylsulfonyl fluoride (1 mM)

Protease inhibitor cocktail, complete (1X; Roche Applied Science)

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  1. doi:10.1101/pdb.rec11848 Cold Spring Harb Protoc 2009: pdb.rec11848-
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