Table 1. Cell numbers, DNA/nucleic acid doses, and transfection volumes suggested for nonviral Magnetofection

Tissue-culture dish	Cell number	DNA quantity (μg)	Volume of vector preparation (mL)	Final transfection volume (mL)
96 well	0.5-2 × 10⁴	0.01-1	0.05	0.2
24 well	0.5-1 × 10⁵	0.1-10	0.1-0.2	0.5-1
12 well	1-2 × 10⁵	0.2-20	0.15-0.3	1.5
Six well	2-4 × 10⁵	0.5-35	0.2-0.6	2-3
60-mm dish	5-10 × 10⁵	1-100	0.5-1.6	5-8
90- to 100-mm dish	10-20 × 10⁵	1-60	0.5-2	10-20
T-25 flask	5-10 × 10⁵	0.2-20	0.5-2	5-10
T-75 flask	20-50 × 10⁵	1-60	0.5-2	15-20

Transfection volume = total transfection volume = volume of vector preparation plus volume of medium covering the cells. As a rule of thumb, nonviral vectors should be prepared in a manner such that the final nucleic acid concentration in the vector preparation does not exceed 100 μg/mL, and preferably does not exceed 50 μg/mL.