Table 2. Cell numbers, magnetic particle doses, and transfection volumes suggested for viral Magnetofection
| Tissue-culture dish | Cell number | Magnetic particles (μg per well) | Volume of vector preparation (mL) | Final transfection volume (mL) |
|---|---|---|---|---|
| 96 well | 0.5-2 × 104 | 0.1-1 | 0.05 | 0.2 |
| 24 well | 0.5-1 × 105 | 1-10 | 0.1-0.2 | 0.5-1 |
| 12 well | 1-2 × 105 | 2-20 | 0.15-0.3 | 1.5 |
| Six well | 2-4 × 105 | 4-40 | 0.2-0.6 | 2-3 |
| 60-mm dish | 5-10 × 105 | 10-50 | 0.5-1.6 | 5-8 |
| 90-100-mm dish | 10-20 × 105 | 30-150 | 0.5-2 | 10-20 |
| T-25 flask | 5-10 × 105 | 10-50 | 0.5-2 | 5-10 |
| T-75 flask | 20-50 × 105 | 30-150 | 0.5-2 | 15-20 |
| Transfection volume = total transfection volume = volume of vector preparation plus volume of medium covering the cells. | ||||
