Protocol

Ant Fat Extraction with a Soxhlet Extractor

  1. Walter R. Tschinkel2
  1. 1 School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA
  2. 2 Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA
  1. ↵3Corresponding author (csmith38{at}asu.edu)

INTRODUCTION

Stored fat can be informative about the relative age of an ant, its nutritional status, and the nutritional status of the colony. Several methods are available for the quantification of stored fat. Before starting a project involving fat extraction, investigators should weigh the advantages and disadvantages of different methods in order to choose the one that is best suited to the question being addressed. This protocol, although not as accurate as some alternatives, facilitates the rapid quantification of many individuals.

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  1. doi:10.1101/pdb.prot5243 Cold Spring Harb Protoc 2009: pdb.prot5243-
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