M-199 incubation medium
Ficoll 400 (Sigma)
Medium 199, modified with Hanks’, L-glutamine (US Biological M2852)
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1. Add 2 g of Ficoll 400 to 100 mL of Medium 199. Mix with a magnetic stir bar until the Ficoll dissolves.
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2. If necessary, adjust the pH to 7.2-7.5 with NaOH.
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3. Filter-sterilize with a Nalgene 115-mL sterile filter unit type S (0.20-μm) or a 10-mL syringe attached to a 0.2-μm syringe filter.
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4. Store refrigerated in labeled 15-mL vials.
After prolonged storage, refilter the medium through a 0.2-μm syringe filter.
