Recipe

In situ hybridization solution (ISHS)

Formamide (50%)

Heparin (50 μg/mL)

Salmon sperm DNA solution (50 μg/mL; e.g., UltraPure; Invitrogen)

SSC (5X; pH 6.5)

SDS (sodium dodecyl sulfate) (0.3%)

Tween 20 (0.1%)

Store at -20°C.

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  1. doi:10.1101/pdb.rec11859 Cold Spring Harb Protoc 2009: pdb.rec11859-
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