Recipe

Paraformaldehyde (4%)

4% paraformaldehyde

1X PBS(P)

0.1% Tween 20

Add 4 g of paraformaldehyde to 100 mL of 1X PBS(P) containing 0.1% Tween 20. Heat without boiling until the paraformaldehyde is completely dissolved. Dispense into 1-mL aliquots and store at −80ºC. Use fresh aliquots only.

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  1. doi:10.1101/pdb.rec11863 Cold Spring Harb Protoc 2009: pdb.rec11863-
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