Recipe

Embryo homogenization buffer

Reagent Final concentration
NaCl 0.1 M
Tris-Cl (pH 8.0) 0.03 M
EDTA 0.05 M
Triton X-100 0.5%
β-Mercaptoethanol 7.7 mM
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  1. doi:10.1101/pdb.rec11867 Cold Spring Harb Protoc 2009: pdb.rec11867-
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