Table 1. Physiological indicators using fluorescence resonance energy transfer

doi:10.1101/pdb.tab1top57

Table 1. Physiological indicators using fluorescence resonance energy transfer

			Wavelength (nm)^a			Maximum emission ratio change^b
Analyte or process	Donor and acceptor^c	ΔFRET^d	Donor excitation	Donor emission	Acceptor emission	Maximum emission ratio change^b	References
Depolarization	Coumarin-phosphatidylethanolamine; bis(thio barbiturate)trimethineoxonol	↓	414	450	560	1.8/(100 mV)	Gonzalez and Tsien (1997)
cAMP	PKA catalytic subunit-FITC; PKA regulatory subunit-TROSu^e	↓	495	520	580	1.6-2.2	Adams et al. (1991, 1993)
Trypsin	BFP-(trypsin-sensitive linker)-GFP	↓	380	445	507	4.6	Heim and Tsien (1996)
Factor X_a	BFP-(factor X_a-sensitive linker)-GFP	↓	385	450	505	1.9	Mitra et al. (1996)
Caspase-3	BFP-(caspase-3-sensitive linker)-GFP	↓	380	440	511	?	Xu et al. (1998)
Ca²⁺-CaM	GFP-CB_SM-BFP	↓	380	448	505	5.7	Romoser et al. (1997)
Ca²⁺	GFP-CB_SM-BFP-CaMCN	↓	380	440	505	1.67	Persechini et al. (1997)
Ca²⁺	ECFP-CaM-M13-EYFP	↑	433	476	527	2.1	Miyawaki et al. (1997, 1999)
Ca²⁺	ECFP-CaM; M13-EYFP	↑	433	476	527	4	Miyawaki et al. (1997, 1999)
β-Lactamase expression	Coumarin-cephalosporin-fluorescein (CCF2)	↓	409	447	520	70	Zlokarnik et al. (1998)

^aDonor excitation wavelength, donor emission wavelength, and acceptor emission wavelength, all in nanometers. Small differences (up to 8 nm) in the wavelengths cited by different laboratories for BFP and GFPs are probably not significant.
^bMaximum factor by which emission ratio changes from zero to saturating levels of the analyte or process, except for depolarization of 100 mV amplitude.
^cHyphens indicate covalent conjugation or fusion. Interacting donor and acceptor molecules are separated by semicolons.
^dFRET indicates whether the efficiency of fluorescence resonance energy transfer is increased (↑) or decreased (↓) by the analyte or process.
^eTetramethylrhodamine N-hydroxysuccinimide.

Table 1. Physiological indicators using fluorescence resonance energy transfer

This Article

Article Category

Services

Personal Folder

Updates/Comments

Citing Articles

Google Scholar

Related Content

Share

Current Issue

From the cover