In Situ Hybridization of Starlet Sea Anemone (Nematostella vectensis) Embryos, Larvae, and Polyps
- Department for Molecular Evolution and Development, Center for Organismal Systems Biology, Faculty of Life Sciences, University of Vienna, 1090 Wien, Austria
- ↵1Corresponding author (ulrich.technau{at}univie.ac.at).
INTRODUCTION
Whole-mount in situ hybridization is used to reveal the spatiotemporal expression pattern of a gene in tissue samples without sectioning the tissue. This protocol describes procedures for fixing starlet sea anemone (Nematostella vectensis) embryos, larvae, and polyps, hybridizing with a digoxigenin (DIG)-labeled RNA probe, and detecting the bound probe.
