Recipe

MOPS-EGTA buffer

Reagent Quantity (for 1 L) Final concentration
MOPS 20.9 g 0.1 M
MgSO4 0.24 g 2 mM
EGTA 0.38 g 1 mM
NaCl 29.2 g 0.5 M
Dissolve the reagents above in 800 mL of diethyl pyrocarbonate (DEPC)-treated H2O. Adjust the pH to 7.5 with NaOH and then add DEPC-treated H2O to give a final volume of 1 L. Sterilize the buffer using a 0.22-μm filter. The buffer can be stored at room temperature for several months.
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  1. doi:10.1101/pdb.rec11933 Cold Spring Harb Protoc 2009: pdb.rec11933-
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