Recipe

PFA-MOPS-EGTA fixation buffer

Reagent Quantity (for 50 mL) Final concentration
Paraformaldehyde 2 g 4% (w/v)
NaOH (1 N) 5 mL
HCl (1 N) 5 mL
MOPS-EGTA buffer to 50 mL
Make fresh. To dissolve paraformaldehyde, add 2 g to 5 mL of 1 NaOH. Heat to 60°C to dissolve, and add 5 mL of 1 N HCl and 35 mL of MOPS-EGTA buffer. Adjust the pH to 7.5 if necessary and then add MOPS-EGTA buffer to give a final volume of 50 mL. Store the buffer at 4°C for several days.
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  1. doi:10.1101/pdb.rec11936 Cold Spring Harb Protoc 2009: pdb.rec11936-
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