Recipe

Hybridization solution (HS)

Reagent Final concentration
Formamide 50% (v/v)
SDS 1% (w/v)
SSC (20X; pH 4.5) 5X
Heparin 0.05 mg/mL
Salmon sperm DNA 0.1 mg/mL
Tween 20 0.1% (v/v)
Prepare HS fresh for each round of in situ hybridization.
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  1. doi:10.1101/pdb.rec11953 Cold Spring Harb Protoc 2009: pdb.rec11953-
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