Recipe

Lysis buffer for DMS treatment

1 mM Tris-Cl (pH 7.5)

400 mM NaCl

2 mM EDTA

0.2% SDS

0.2 mg/mL proteinase K

Prepare immediately before use.

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  1. doi:10.1101/pdb.rec11961 Cold Spring Harb Protoc 2009: pdb.rec11961-
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