Recipe

First-strand buffer (5X)

200 mM NaCl

50 mM Tris-Cl (pH 8.9)

25 mM MgSO4

0.05% gelatin

Store at −20°C.

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  1. doi:10.1101/pdb.rec11965 Cold Spring Harb Protoc 2009: pdb.rec11965-

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