Recipe

Restriction enzyme digestion buffer

10 mM Tris-Cl (pH 7.4)

50 mM NaCl

10 mM MgCl2

0.2 mM EDTA

0.2 mM EGTA

0.15 mM spermine

0.5 mM spermidine

1 mM β-mercaptoethanol

Store at 4°C.

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  1. doi:10.1101/pdb.rec11967 Cold Spring Harb Protoc 2009: pdb.rec11967-
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