Dissection and Fixation of Rabbit Embryos

Bernd Püschel; Christoph Viebahn

doi:10.1101/pdb.prot5351

Protocol

Dissection and Fixation of Rabbit Embryos

Bernd Püschel and
Christoph Viebahn1

Department of Anatomy and Embryology, University of Göttingen, 37075 Göttingen, Germany

↵¹Corresponding author (cviebah{at}gwdg.de).

INTRODUCTION

This protocol describes general methods for dissection and fixation of rabbit embryos. Depending on the investigation, embryos can be dissected either alive or after fixation. Some embryonic stages are surrounded by an envelope, the zona pellucida, which might have to be removed for some applications (e.g., immunohistochemistry). Fixation methods vary according to different experimental needs, such as structure preservation (morphology), antigen preservation (immunoreactivity), or mRNA accessibility (in situ hybridization).