PHEM (5X)
50 mM EGTA
125 mM HEPES
10 mM MgCl2
300 mM PIPES
Adjust the pH to 6.9 with NaOH. This stock solution (Schliwa M, van Blerkom J. 1981. Structural interaction of cytoskeletal components. J Cell Biol 90: 222-235) is stable for several months stored at 4°C.
