Wheat grass stock solution

MATERIALS

Reagents

Wheat grass powder (Pines International, Inc.)

Bio Herbe de Blé (l’Arbre de Vie) can also be used but is not suitable for rich Paramecium culture medium.

Water, Millipore-filtered

Equipment

Autoclave

Filter, cotton gauze

Flask, glass, 1-L

Flasks, glass, 200-mL

Funnel

Hot plate

METHOD

• 1. Add 50 g of wheat grass powder to 750 mL of Millipore-filtered water in a glass flask.

• 2. Boil for 15 min.

• 3. Filter the suspension through a thick layer of cotton gauze. Press to recover as much fluid as possible.

• 4. Adjust the volume to 1 L with Millipore-filtered water. Distribute the medium into five 200-mL flasks.

• 5. Autoclave for 20 min at 120°C.

  Store the sterilized stock solution for weeks at 4°C-5°C.