Paramecium culture medium
MATERIALS
Reagents
β-sitosterol (4 mg/mL, prepared in 100% ethanol)
Store in the dark at 4°C.
Equipment
Incubator preset to 27°C or 35°C (see Step 2)
Inoculation loop
METHOD
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1. Inoculate wheat grass medium (standard or rich, as appropriate for the intended experiment) with fresh bacteria.
Paramecium culture medium is termed standard or rich, depending on whether it is made with standard or rich wheat grass medium.
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2. Incubate without shaking for 12-18 h (i.e., overnight) at 27°C.
If Bio Herbe de Blé was used in the standard wheat grass medium, incubate at 35°C.
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3. Immediately before use, add β-sitosterol to a final concentration of 0.8 μg/mL.
