Recipe

LBAT-agar plates

Ampicillin stock solution, filter-sterilized (100 mg/mL)

LB agar

Tetracycline stock solution, filter-sterilized (12.5 mg/mL)

Autoclave 1 L of LB agar. Cool to 55°C. Add 1 mL of ampicillin stock (to a final concentration of 100 μg/mL) and 1 mL of tetracycline stock (to a final concentration of 12.5 μg/mL). Pour into Petri dishes (~25 mL/100-mm plate).

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  1. doi:10.1101/pdb.rec12081 Cold Spring Harb Protoc 2010: pdb.rec12081-

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